Presentation | July 15, 2011

Presentation: UPLC In Pharmaceutical Process Development - Comparison With Theoretical Promise

Source: Pittcon 2015

The effect of extra-column volume on performance can be significant in UPLC although the system was optimized for fast separation.

Columns with small internal diameters and / or short column lengths are more susceptible to extra-column band-broadening for high- speed separation in UPLC.