Presentation: Method Transfer Between HPLC And UHPLC: Issues And Solutions

A natural requirement for UHPLC instruments is to have minimized gradient delay and peak dispersion. Systems are typically designed for the operation of small bore columns (2.1 mm-3.0 mm internal diameter) packed with small particles (1.8 µm) to achieve highest separation efficiencies. With regard to solvent delivery, these systems usually differ significantly from standard HPLCsystems. The most obvious aspect is the dwell volume of such UHPLC systems, which is purposely minimized to allow fast gradient separations.